闽清县杂交中稻新品种比较试验

为筛选适宜闽清县推广的杂交中稻品种,进行了杂交中稻新品种比较试验。结果表明,两优616、嘉浙优99、中浙优8号比对照增产,主要农艺性状综合表现较好,可以作为当地主要推广的新品种,简述了表现较好品种的产量及农艺性状。     

Effect of Plasticizers on Properties of Rice Straw Fiber Film

In order to improve the properties of rice straw fiber film, one factor contrast test method was employed. Plasticizer type was chosen as input variable, dry tension strength and elongation, wet tension strength and elongation, bursting strength and tearing strength were chosen as indexes. The results showed that there were significant differences among the means of dry tension strength, dry elongation and bursting strength of different plasticizers; there were not significant differences among the means of wet tension strength, wet elongation and tearing strength of different plasticizers; for dry tension strength and elongation, glycerol had a significant difference with sorbitol and PEG, no significant difference was observed between sorbitol and PEG, dry tension strength added glycerol had been reduced 6.8% compared with that added sorbitol, reduced 9.5% compared with that added PEG; elongation had been improved 6.1% and 9.4%, respectively; for bursting strength, sorbitol had a significant difference with glycerol and PEG, no significant difference was observed between glycerol and PEG; bursting strength added glycerol and added PEG had been improved 6.9% and 5.6%, respectively compared with that of the added sorbitol. The results provided a theoretical reference for further improving the straw fiber film manufacturing process.     

苎麻单纤维断裂强力统计特征研究

为了研究苎麻单纤维断裂强力的全貌与分布规律,通过采用等速伸长法测得其断裂强力,根据断裂强力的频数直方图的分布趋势,用正态分布的偏度、峰度等参数对其进行分析,用Q-Q图与Kolmogorov-Smirnov（以下简称K-S）检验法检验苎麻单纤维强力的正态性,总体上服从正态分布规律,但有一定偏差,不对称,为右偏尖峰态。同时提出了建立在统计基础上的苎麻单纤维断裂强力指标体系,即运用主体断裂强力、品质断裂强力、平均断裂强力、弱强纤维断裂强力和超强纤维强断裂力五个指标来表征单纤维断裂强力。     

催化器OBD诊断监测频率功能的研究与验证

介绍了利用储氧量(OSC)法对催化器进行OBD故障诊断的原理,并介绍了法规新增的监测频率(IUPR)功能。通过转鼓试验,验证了在NEDC,FTP75和JC08循环中催化器诊断的完成情况和相应的IUPR率的增长情况。通过实际道路试验,观察IUPR率的变化规律,我们得出:经过一定里程后,同一OBD系族车型催化器诊断的IUPR率趋于稳定一致,可以作为该车型在用符合性检查的数据指标。     

Advances in DUS Test Technique for Coconut

As great progress has been made in the field of protection of new plant varieties, more attention is paid to the standardization of DUS （Distinctness, Unifor- mity, and Stability） test procedure. For further studies of tropical plants as their im- portance in agriculture and germplasm, protection of coconut becomes more signifi- cant and thus DUS test technique of coconut is needed. In this essay, we analyzed the status quo of the DUS test guidelines by International Union for the Protection of New Varieties of Plants （UPOV proj.3） and national DUS test guidelines in Chi- na, and provided some suggestions or promotions for improving the guidelines of DUS test in coconut.     

规模化养鸡场A型产气荚膜梭菌的分离鉴定及其对海南霉素敏感性的测定

为探究海南霉素对产气荚膜梭菌的敏感性,在某大型养鸡场采集了209份鸡肠道样本,采用选择性培养基和MALDI-TOF质谱仪微生物鉴定仪鉴定,并对鉴定的菌株进行分型。采用琼脂稀释法测定了海南霉素、恩拉霉素、盐霉素、丁酸甘油酯对临床分离的产气荚膜梭菌的抗菌活性,以期为当前限抗禁抗后养殖场选择合适的药物控制产气荚膜梭菌提供理论依据。试验结果表明：共分离出61株产气荚膜梭菌,分离率为29.2%;通过多重PCR对所分离的产气荚膜梭菌进行分型,结果分离株均为A型产气荚膜梭菌;4种药物均有不同程度的抑菌效果,其中海南霉素与恩拉霉素对鸡源产气荚膜梭菌的抑菌效果最好,且MIC值范围均为0.004～0.06μg/mL。海南霉素具有很好的抗梭菌效果。     

Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation

Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.     

Detecting endotoxin activity in bovine serum using an automated testing system

The aim of the present study was to compare the ability of the commercially available portable test system (PTS^TM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS^TM was not significantly different from that of the traditional LAL-based assays. The results using PTS^TM correlated with those using KT (r²=0.963, P<0.001) or KC assays (r²=0.982, P<0.001). Based on these findings, the PTS^TM could be applied as a simplified system to assess endotoxin activity in bovine serum.